siRNA / RNAi /miRNA transfection Rat A10 (Vascular smooth muscle cells)

Get tips on using Smooth Muscle Actin Antibody (B4): sc-53142 to perform Immunohistochemistry Mouse - SMA

Get tips on using Anti-alpha smooth muscle Actin antibody (ab5694) to perform Immunohistochemistry Mouse - SMA

Get tips on using anti-alpha-Smooth Muscle Actin mouse monoclonal, ASM-1 to perform Immunohistochemistry Alpha smooth muscle Actin - Mouse -NA- -NA-

Get tips on using FlashTag™ Biotin HSR RNA Labeling Kits to perform Microarray RNA amplification & Labeling - Rat primary vascular smooth muscle cells Biotin

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using SmGMTM- 2 Smooth Muscle Cell Growth Medium -2 BulletKitTM to perform Mammalian cell culture media HCASMC

Get tips on using SmGMTM- 2 Smooth Muscle Cell Growth Medium -2 BulletKitTM to perform Mammalian cell culture media HCtASMC

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat aortic smooth muscle cells (rASMC)

Get tips on using Monoclonal Mouse Anti-Human Actin (Smooth Muscle) (Concentrate) Clone 1A4 to perform Immunohistochemistry Mouse - SMA

Get tips on using pGL3-Basic Vector to perform Reporter gene assay luciferase - vascular smooth muscle cells (VSMC)